Covalent Chromatography
Abstrakt
Covalent chromatography is an important, highly specific separation technique. In covalent chromatography, proteins and other ligands bearing accessible thiol groups form a mixed disulfide with the gel. The proteins are thus covalently linked to the stationary phase from which they can be subsequently eluted by addition of a reducing agent. Basic principles of the method are given together with the methods for the synthesis of gels. In addition, the paper describes several special applications of covalent chromatography.Stahování
Publikováno
15.06.2000
Jak citovat
Glatz, Z. (2000). Covalent Chromatography. Chemické Listy, 94(5). Získáno z http://www-.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/2512
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